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| Coe College | Iowa | Cedar Rapids | Midwest | |
| Drake University | Iowa | Des Moines | Midwest | |
| Emporia State University | Kansas | Emporia | Midwest | |
| Iowa State University | Iowa | Ames | Midwest | http://sites.google.com/site/isutriclub/ |
| Marquette University | Wisconsin | Milwaukee | Midwest | |
| Minnesota State University | Minnesota | Mankato | Midwest | http://mnsutriclub.webstarts.com/index.html/ |
| St. Olaf College | Minnesota | Northfield | Midwest | |
| University of Iowa | Iowa | Iowa City | Midwest | http://www.trihawks.com/ |
| University of Minnesota | http://www.umntriteam.com | |||
| University of Missouri | Missouri | Columbia | ||
| University of Wisconsin- Eau Claire | Eau Claire | |||
| University of Wisconsin - La Crosse | La Crosse | http://www.uwlax.edu/recsports/club_sports_triathlon.html | ||
| University of Wisconsin-Madison | http://www.uwtriathlon.com | |||
| University of Wisconsin - Milwaukee | http://www.uwmtriathlon.blogspot.com | |||
| Wartburg College | ||||
| Washington University | Missouri | St. Louis | http://www.washutri.com |
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